| Clin Immunol Immunopathol. 1996 Aug;80(2):123-8. |
|
Monocyte chemoattractant
protein-1 in chronic proliferative immune complex
nephritis.
Moxey-Mims MM, Nielsen L, Noble B, Lwebuga-Mukasa
JS.
Department of Pediatrics, State University of New
York at Buffalo 14222,
In rats with chronic serum sickness, proliferative
immune complex glomerulonephritis progresses in
three discrete stages, designated mild, moderate, and severe. One distinguishing
immunopathologic feature, the progressive increase in the
number of glomerular macrophages, is closely correlated
with decreasing kidney function. We hypothesized that monocyte chemoattractant protein-1,
a beta-subfamily chemokine with potent monocyte-specific chemotactic activity,
might contribute to this macrophage accumulation. Immunohistochemical
methods were used to identify monocyte chemoattractant protein-1 in kidney tissue sections. Total
RNA was extracted from the kidneys of rats at each stage of chronic serum
sickness, and age-matched controls, and Northern blot analysis was performed
with a rat monocyte chemoattractant
protein-1 cDNA probe. Tissue staining localized
monocyte chemoattractant protein-1
to the glomerular capillary wall and mesangium in chronic serum sickness. Minimal quantities of
monocyte chemoattractant protein-1
mRNA were detected in the kidneys of normal control rats, with marked increases
in mRNA as chronic serum sickness nephritis progressed to the moderate stage.
There was then an apparent decrease in monocyte
chemoattractant protein-1 mRNA in the severe stage.
The degree of protein staining and mRNA levels paralleled each other. We conclude
that monocyte chemoattractant
protein-1 is a potentially important chemotactic
agent in chronic serum sickness nephritis.
PMID: 8764556 [PubMed
- indexed for MEDLINE]